Fluorescent Probes for Biological Imaging

Fluorescent Probes for Biological Imaging

Owing to the high sensitivity, high resolution, and the wealth of contrast mechanisms, fluorescence imaging is the most versatile and widely used visualization modality to study the structure and function of biological systems and the molecular process in living organisms without perturbing them. Fluorescence molecular imaging is an evolving field of imaging sciences, which involves the develop...

Nucleic acid fluorescent probes for biological sensing.

Nucleic acid fluorescent probes are playing increasingly important roles in biological sensing in recent years. In addition to the conventional functions of single-stranded DNA/RNA to hybridize with their complementary strands, affinity nucleic acids (aptamers) with specific target binding properties have also been developed, which has greatly broadened the application of nucleic acid fluoresc...

Fluorescent Probes for Multiplexed Intracellular Imaging

The focus of our center is to design, prepare, and investigate novel fluorescent probes, termed “through-bond energy transfer cassettes”, for intracellular imaging. At this stage, it involves chemical syntheses of the probes (Burgess), conjugation to proteins involved in a signaling cascade for nuclear localization, and observation in cells, both under conventional conditions (Schroeder) and on...

Smart fluorescent probes for imaging macrophage activity.

Macrophages are multi-functional immune cells with key roles in host defense and tissue remodelling. The broad array of macrophage functions has prompted the development of very diverse smart fluorescent architectures, rationally designed to elicit a fluorescent signal only after target engagement. This tutorial review covers recent advances in the design, synthesis and application of smart flu...

Quantum Dots-Based Biological Fluorescent Probes for In Vitro and In Vivo Imaging